User blog comment:763492/Hatsune Miku on Hollywood's Walk of Fame/@comment-25577599-20141025005813/@comment-5665682-20141025131132

@Dizzy I think that was exactly the problem. People expected something "better" than the thing already happened. Gosh, there were some people believing Letterman would "interview" Miku. And is notorious that there's a lot of "new-to-vocaloid" people that also disliked Miku, and, in consequence, Vocaloid. Though the video has over 1 million views, you don't know how many of those views were made by people that do like Miku. And, even if it doesn't has a general dislike ratio, fans will bury aaall this like Gaga's concert. Nobody will talk about this anymore in a long time. And lurking through the amount of comments, negative comments, you can also see a lot of people writing "so, this is vocaloid?, what the heck is horrible" and stuff.

So, yes, there's a reason to believe that Miku in Letterman do affected the vision about vocaloid and Miku herself. In a small percentage, but it affected.