This song describes Miku introducing herself to the world. It is a denpa song, and thus the title Miku Miku ni Shite Ageru (I'll make you Miku-Miku-ed) carries no specific meaning in Japanese. Thus, what the title actually means is a subject of disagreement among fans. However, the term was derived from ぼこぼこにしてあげる (boko boko ni shite ageru), which means I'll beat you up. One of the prevailing interpretations among the Japanese fandom is I'll make you obsessed with Miku, conveying the idea that Miku strikes fans' hearts with her voice. Similar expressions are みくみくにされる (Miku Miku ni sareru) (I'm Miku-Miku-ed), フルみっくにされる (furu Mikku ni sareru) (I'm Miku-Miku-ed to the full extent) and so on). It is commonly said to newcomers in the VOCALOID fandom as a welcome, or to make them a VOCALOID fan by replacing Miku with another VOCALOID name, implying that they will turn you into an overall VOCALOID fan.
The song was recorded as the first Vocaloid song to reach 1,000,000+ views on Nico Nico. The song also topped the first Weekly Vocaloid Ranking on Nico Nico. It was one of three early Miku songs to establish Miku's character as it is known today, along with kz's "Packaged" and "Koisuru VOC@LOID". As of 2018, "Miku Miku ni Shite Ageru" is officially the most viewed Miku song (original upload) on Nico Nico, with over 13 million views. The next most popular original Vocaloid upload is Kurousa's Senbonzakura, with 12 million views.
A new version of the song for 2012 using Miku's Append. This new version is longer, including new lyrics and a visually attractive PV that shows to Miku in her multiple facets, representations and derivatives. It has reached over 1,000,000 views and is featured on the album Heartsnative.
Animelo is an Anime-Central concert where songs from various anime are performed in a concert-like fashion. It was here that Miku appeared as a surprise in 2009. Not only Miku came, but Rin and Len's voice provider, Asami Shimoda, sang Kokoro as a teaser for introducing Miku, who sang "Miku Miku ni Shite Ageru♪". The song Black★Rock Shooter followed Miku's appearance, along with its anime's trailer.